HTS and post-screen hit validation

Each HTS project is unique and will be managed, scheduled, and run by the core director (kratia@uic.edu) or in collaboration with the PI's lab. We require an initial consult for all new projects. Examples of high-throughput assays run by our facility include:

Biochemical assays:

  • Protein-protein interactions (Alphascreen)
  • Enzyme-based assays-- timecourse and endpoint measurements (fluorescence intensity, TR-FRET, absorbance)
  • Protein-ligand (fluorescence polarization)

Cell-based assays:

  • Reporter gene assays (fluorescence intensity, luminescence, absorbance)
  • Cell viability assays (luminescence)
  • Imaging (high-content)